Extending the useful life span of DNA probes for fluorescence in situ hybridization.
نویسندگان
چکیده
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In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
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عنوان ژورنال:
- BioTechniques
دوره 26 6 شماره
صفحات -
تاریخ انتشار 1999